Characterization of 24 microsatellite markers in Primula chungensis (Primulaceae), a distylous–homostylous species, using MiSeq sequencing
نویسندگان
چکیده
منابع مشابه
Characterization of polymorphic microsatellite markers for Primula sikkimensis (Primulaceae) using a 454 sequencing approach1
PREMISE OF THE STUDY Microsatellite markers from Primula sikkimensis (Primulaceae) were developed for testing deep lineage divergence and speciation events. METHODS AND RESULTS A total of 3112 microsatellites were identified from 61,755 unique reads though 454 pyrosequencing technology. Twenty-nine microsatellite loci were selected for PCR amplification and polymorphic analyses. Among the 29 ...
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Primula poissonii (Primulaceae) is a perennial herb, widely distributed in the Hengduan Mountain region of Southwest China. In this study, Roche 454 pyrosequencing was used to isolate microsatellite markers. A total of 4528 unique sequences were identified from 68,070 unique reads. Of these, eighty-seven microsatellite loci were screened for utility using two criteria: successful PCR amplificat...
متن کاملDevelopment of 13 microsatellite markers in the endangered Sinai primrose (Primula boveana, Primulaceae)1
UNLABELLED PREMISE OF THE STUDY We developed microsatellite markers for the endangered plant Primula boveana, the Sinai primrose, and assessed the cross-transferability of these markers to six related taxa. • METHODS AND RESULTS DNA sequences containing microsatellites were isolated from a microsatellite-enriched library. We obtained successful amplification of 13 microsatellite primer pai...
متن کاملDevelopment of microsatellite markers using Illumina MiSeq sequencing to characterize Ephedra gerardiana (Ephedraceae)1
PREMISE OF THE STUDY Ephedragerardiana (Ephedraceae), occurring in the Himalayan ranges, is an important plant species used in Tibetan medicine. Due to the lack of molecular markers to characterize genetic diversity, knowledge for conservation and uses of E. gerardiana resources is limited; we therefore developed microsatellite markers for use in this species. METHODS AND RESULTS Using Illumi...
متن کاملDevelopment of 12 polymorphic microsatellite loci in the high alpine perennial Primula halleri (Primulaceae)1
UNLABELLED PREMISE OF THE STUDY Primula halleri is a high alpine endemic species with a patchy distribution in Central and Eastern European mountains. Little is known about the structure of genetic variation and mating system in populations of this species. • METHODS AND RESULTS We report on the development of 12 novel, polymorphic microsatellite loci for P. halleri. Tests for amplificatio...
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ژورنال
عنوان ژورنال: Plant Diversity
سال: 2016
ISSN: 2468-2659
DOI: 10.1016/j.pld.2015.12.002